Prevalence of Hepatitis B & C
in Volunteer & Exchange
Donors
Dr.Shahida Baloch
Introduction
 Hepatitis is term to describe a nonspecific liver
inflammation Until now are known 8 types of
hepatitis: A,...
Introduction (Cont’d)
 Hepatitis B virus infection (HBV) is
responsible for an estimated 1-2 million deaths
worldwide eve...
 One of the most common route of transmission of
hepatitis B and C is blood transfusion but it cannot be
considered the o...
Aim of study
 To analyze the prevalence of HBsAg and anti-
HCV antibodies in volunteer and exchange
blood donors
Material And Methods
 A total of 18241donors were screened for
hepatitis B and hepatitis C, using Architect,
CMIA method ...
 Selection of donors
Healthy individuals aged between 18-55 years
of age without any previous history of
jaundice, tuberc...
Specimen collection and preparation
 Fresh blood specimens were collected in EDTA tubes from the
donors for processing
 ...
Screening tests performed
during July 2009 to June
2010
HBsAg Reactive HCV Reactive
Exchan
ge
donor
(A)
Volunt
ary
donor
(...
Results
(July 2009 to April 2010)
 Total screening tests performed in both
exchange & voluntary donors during
 HBsAg pos...
% of HBsAg Reactive % of HCV Reactive
In
Exchange
donor
In
Voluntary
donor
Both
exchange &
voluntary
donors
In
Exchange
do...
Conclusion
 Blood is one of the main sources of transmission of
Hepatitis B and Hepatitis C. Donor selection is of
paramo...
THANK YOU
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Prevalance of h bs ag,hcv and hiv

prevalence of hepatitis B, c and HIV in blood donors
Published on: Mar 4, 2016
Published in: Healthcare      Health & Medicine      Business      
Source: www.slideshare.net


Transcripts - Prevalance of h bs ag,hcv and hiv

  • 1. Prevalence of Hepatitis B & C in Volunteer & Exchange Donors Dr.Shahida Baloch
  • 2. Introduction  Hepatitis is term to describe a nonspecific liver inflammation Until now are known 8 types of hepatitis: A, B, C, D, E, F, G,H.  Hepatitis B and C are similar types of liver infection, which are mostly spread through blood and blood products.
  • 3. Introduction (Cont’d)  Hepatitis B virus infection (HBV) is responsible for an estimated 1-2 million deaths worldwide every year and around 300 millions are carriers for HBV out of which around 80% reside in Asia  In Pakistan the rate of HBV and HCV infection ranges from 8- 15% in general population with variations in the frequency and characteristics in various parts of the country
  • 4.  One of the most common route of transmission of hepatitis B and C is blood transfusion but it cannot be considered the only source of spread of hepatitis viruses as at maximum only 1% of general population receives blood transfusion in a year  Some of the patients are dependent on blood transfusions such as patients suffering from haematological disorders requiring replacement therapy, patients undergoing major surgical procedures & getting haemodialysis & patients receiving organ transplants.
  • 5. Aim of study  To analyze the prevalence of HBsAg and anti- HCV antibodies in volunteer and exchange blood donors
  • 6. Material And Methods  A total of 18241donors were screened for hepatitis B and hepatitis C, using Architect, CMIA method (Abbott, USA)  The study was conducted at Fatimid Foundation Blood Bank & Haematological Services and analyzed period was from July 2009 – April 2010
  • 7.  Selection of donors Healthy individuals aged between 18-55 years of age without any previous history of jaundice, tuberculosis, heart, lung or kidney disease, prolonged fever, convulsions etc., and no history of major surgery, dental procedures, transfusion in the past 6 months, or blood donation during last three months and not receiving any drugs or current medication
  • 8. Specimen collection and preparation  Fresh blood specimens were collected in EDTA tubes from the donors for processing  Specimens were handled gently to avoid haemolysis of blood  Plasma was separated by centrifugation for 20 minutes initially at 3500 rpm (Labofuge, Heraeus, Germany)  For confirmation of Borderline/Gray zone results, two tubes are made from the Plasma collected from the suspected bag & centrifuged again at 10,000 rpm (Heraeus Germany) for 20 minutes to remove most of the medium weight interfering substances
  • 9. Screening tests performed during July 2009 to June 2010 HBsAg Reactive HCV Reactive Exchan ge donor (A) Volunt ary donor (B) Total Test Perform ed C= A+B In Exchan ge donor a1 In Voluntary donor b1 Total C1= a1+b1 In Exchan ge donor a1 In Volunta ry donor b1 Total c1= a1+b1 4532 13709 18241 99 455 554 143 518 661
  • 10. Results (July 2009 to April 2010)  Total screening tests performed in both exchange & voluntary donors during  HBsAg positive in Voluntary donors  HbsAg positive in Exchange donors  HCV positive in Voluntary donors  HCV positive in Exchange donors 18241 455 (3.31%) 99 (2.18%) 518 (3.77%) 143 (3.15%)
  • 11. % of HBsAg Reactive % of HCV Reactive In Exchange donor In Voluntary donor Both exchange & voluntary donors In Exchange donor In Voluntary donor Both exchange & voluntary donors 2.18% 3.31% 3.03% 3.15% 3.77% 3.62%
  • 12. Conclusion  Blood is one of the main sources of transmission of Hepatitis B and Hepatitis C. Donor selection is of paramount importance. Majority of blood donors in our country are exchange (replacement) donors or voluntary donors. They are usually healthy peoples without any apparent illness. Our study shows that the seroprevalence of Hepatitis B and C among exchange and volunteer blood donors is slightly higher than exchange donors, because in voluntary donors both one time & regular donors are included. Further studies are required to separate regular and one time voluntary donors.
  • 13. THANK YOU

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