IN THE NAME OF GODIN THE NAME OF GOD
Nanoparticles in modernNanoparticles in modern
separation scienceseparation science
Nano science
Nanotechnology is science, engineering, and technology conducted at the
nanoscale , which is about 1 to 100 n...
Nanoparticle applications in modern
separation science
Use of NPs in:
•Capillary electrophoresis
•Open tubular capillary e...
The Potential of NPs in separation
science
1. NPs are relatively easy to synthesize.
2. NP size is carefully controlled du...
The Potential of NPs in separation science
5.Several Kinds of NP and nanostructure have been applied to separation
science...
The Potential of NPs in separation science
1. These applications are mainly reported in academic research
Laboratories ,wh...
Features of Nano particles in analytical
chemistry
NPs have been used in analytical chemistry
•Mono functional NPs : have ...
Some of the characteristic chemical features of a
monolayer-protected gold NP(AuNP)colloidal solution
The characteristic s...
Some of the characteristic chemical features of
a monolayer-protected gold NP (Au NP)
colloidal solution
E.Guihen,TrendsAn...
A multifunctional NP
E.Guihen,TrendsAnal.Chem.46(2013)1-14
10
A multifunctional NP, which can be doped with a different NP...
Liquid chromatographyLiquid chromatography
E.Guihen,TrendsAnal.Chem.46(2013)1-14 11
• In LC,NPs have been used less extens...
Liquid chromatographyLiquid chromatography
E.Guihen,TrendsAnal.Chem.46(2013)1-14 12
Chromatograms of a test mixture obtain...
LiquidLiquid chromatographychromatography
• In 2006 Kobayashi et al reported three kinds of thiol-modified AuNP-coated
pol...
Liquid chromatographyLiquid chromatography
(1) pyridine;(2)o-methylaniline;(3)N-methylaniline;and,(4)N,N-dimethylaniline
•...
Liquid chromatographyLiquid chromatography
• Dun et al. Developed a zirconia-based LC packing material,ZrO2/SiO2, which
co...
Gas chromatographyGas chromatography
• GC has seen even less activity in relation to the use of NP stationary phases.
Perh...
Gas chromatographyGas chromatography
• Na et al developed a ionic liquid-dispersed silica NPs(IL-SN)capillary column,
Comb...
Gas chromatographyGas chromatography
E.Guihen,TrendsAnal.Chem.46(2013)1-14 18
GC chromatograms of mixtures of volatile(A)a...
MicrochipMicrochip electrophoresiselectrophoresis
E.Guihen,TrendsAnal.Chem.46(2013)1-14 19
For protein, DNA, RNA and other...
SeparationSeparation mechanismmechanism of the electrophoreticof the electrophoretic microchipmicrochip systemsystem
E.Gui...
Microchip electrophoresisMicrochip electrophoresis
Liang et al developed for the modification of a poly (dimethylsiloxane)...
Types of Columns
E.Guihen,TrendsAnal.Chem.46(2013)1-14
22
Capillary Electrophoresis And Open Tubular capillary electro
chromatography
• NPs use in OTCEC as inner wall coatings or p...
Capillary Electrophoresis And Open Tubular capillary electro
chromatography
• Yang et al used cyclodextrin-modified AuNPs ...
Capillary Electrophoresis And Open Tubular capillary electro
chromatography
• Qu et al investigated the use of an octadecy...
Ion chromatographyIon chromatography
Monolithic columns are frequently used in separation science
Cao and co-workers devel...
Ion chromatographyIon chromatography
Surface modifications of a single poly (glycidyl methacrylate-co-ethylene dimethacryl...
Ion chromatographyIon chromatography
E.Guihen,TrendsAnal.Chem.46(2013)1-14 28
Separation of proteins in ion exchange mode ...
29
of 29

Nanoparticles in modern separation science

Nanoparticles in modern separation science
Published on: Mar 3, 2016
Published in: Science      
Source: www.slideshare.net


Transcripts - Nanoparticles in modern separation science

  • 1. IN THE NAME OF GODIN THE NAME OF GOD Nanoparticles in modernNanoparticles in modern separation scienceseparation science
  • 2. Nano science Nanotechnology is science, engineering, and technology conducted at the nanoscale , which is about 1 to 100 nanometers. nanotechnology are the study and application of extremely small things and can be used across all the other science fields , such as chemistry, biology, physics ,materials science , and engineering. E.Guihen,TrendsAnal.Chem.46(2013)1-14 2
  • 3. Nanoparticle applications in modern separation science Use of NPs in: •Capillary electrophoresis •Open tubular capillary electro chromatography •Liquid chromatography •Gas chromatography •Ion chromatography •Microchip electrophoresis E.Guihen,TrendsAnal.Chem.46(2013)1-14 3
  • 4. The Potential of NPs in separation science 1. NPs are relatively easy to synthesize. 2. NP size is carefully controlled during chemical using the reaction conditions (e.g. , temperature ,pressure , and solution pH). 3. NPs differ in their chemical and physical properties from the parent bulk-chemical material 4. NPs can be functionalized by a wide range of different chemistries , with amino(NH2), thiol(SH)and carboxyl(COOH). E.Guihen,TrendsAnal.Chem.46(2013)1-14 4
  • 5. The Potential of NPs in separation science 5.Several Kinds of NP and nanostructure have been applied to separation science , including fullerenes, nanotubes ,silica, polymers, zeolite ,lipids ,latex , metal oxides , and Silver NPs(Ag NPs)and Au NPs 6.AuNPs are the most popular in separation science,due to their inert nature ,ease of functionality with thiol groups 7.NPs display a large surface area-to-volume ratio,which is ideal for low mass transfere in chromatography .This feature is also invaluable in miniaturized electro-migration techniques (e.g.,MCE) E.Guihen,TrendsAnal.Chem.46(2013)1-14 5
  • 6. The Potential of NPs in separation science 1. These applications are mainly reported in academic research Laboratories ,where separation success has been greater than or equivalent to current state-of-the-art chromatographic and electrophoretic methods . 2. In spite of this Success ,this technology has not penetrated industrial chemistry laboratories (e.g., pharmaceuticals and forensics). 3. In some cases ,these small particles have offered extraordinary benefits in terms of separation efficiency , selectivity and resolution E.Guihen,TrendsAnal.Chem.46(2013)1-14 6
  • 7. Features of Nano particles in analytical chemistry NPs have been used in analytical chemistry •Mono functional NPs : have been the selected choice and have primarily been used in analytical chemistry to provide a single function •Multi functional NPs : can achieve a multi or mixed effect using one system and applications to in medical imaging and drug delivery , could hold great potential in the separation of complex biological sample ,as many analytes may be monitored simultaneously E.Guihen,TrendsAnal.Chem.46(2013)1-14 7
  • 8. Some of the characteristic chemical features of a monolayer-protected gold NP(AuNP)colloidal solution The characteristic strong wine-red color is apparent E.Guihen,TrendsAnal.Chem.46(2013)1-14 8
  • 9. Some of the characteristic chemical features of a monolayer-protected gold NP (Au NP) colloidal solution E.Guihen,TrendsAnal.Chem.46(2013)1-14 9 )TEM)Shows the solution consists of spherical particles with an average size of 5nm Visible spectrum shows Weak plasmon -resonance absorption maxima in the range )490–510nm)with λmax of 501nm,which further confirms the nanometer)nm)range of the Au NPs
  • 10. A multifunctional NP E.Guihen,TrendsAnal.Chem.46(2013)1-14 10 A multifunctional NP, which can be doped with a different NP and/or a dye.
  • 11. Liquid chromatographyLiquid chromatography E.Guihen,TrendsAnal.Chem.46(2013)1-14 11 • In LC,NPs have been used less extensively due the high back pressure that would be generated coupled with frictional heating effects. LC instrumentation is not designed to with stand the ultra high pressures that a nano -sized packed bed would generate. • In 2002 , Cintron and Colon slurry-packed organo -silica NPs into fused silica capillaries )50-µm internal diameter),which were then tested in ultra high pressure LC )UHPLC).
  • 12. Liquid chromatographyLiquid chromatography E.Guihen,TrendsAnal.Chem.46(2013)1-14 12 Chromatograms of a test mixture obtained at pressures of (left) 5000 psi and (right)50,000psi1)ascorbic acid;(2)hydroquinone;(3)resorcinol;(4)catechol (5) 4-methylcatechol. dp = 670nm.UVdetectionat215nm. Fused silica packed column . Column included stability underextreme acidic (pH<1)and basic (pH>11) conditions Short analysis times and High separation efficiencies)500,000plates/m) for LC Pressure LC(UHPLC).
  • 13. LiquidLiquid chromatographychromatography • In 2006 Kobayashi et al reported three kinds of thiol-modified AuNP-coated polystyrene packing for LC . Compared to the octadecyl silica(ODS)column , different selectivities for aromatic compounds were observed using this column , indicating that this kind of novel column could be used to achieve unique separation for different samples. • Wu et al.reported a novel core-shell composite[ SiO2-nLPD (liquid-phase deposition)], consisting of µm-sized silica spheres as a core and nm-sized titania particles as a surface coating, were prepared by LPD. • The benefit of last method:The benefit of last method: E.Guihen,TrendsAnal.Chem.46(2013)1-14 13
  • 14. Liquid chromatographyLiquid chromatography (1) pyridine;(2)o-methylaniline;(3)N-methylaniline;and,(4)N,N-dimethylaniline • the work showed that the core-shell composite can efficiently separate phosphate compounds due to the Lewis acid-base interaction between titania and phosphate group ,when used as LC packing E.Guihen,TrendsAnal.Chem.46(2013)1-14 14
  • 15. Liquid chromatographyLiquid chromatography • Dun et al. Developed a zirconia-based LC packing material,ZrO2/SiO2, which consisted of µm-sized silica spheres as core and nm-sized zirconia particles as a surface coating , formed by layer-by-layer self assembly . The support ,ZrO2/SiO2, and the stationary-phase,C18 bonded ZrO2/SiO2 , exhibited excellent chemical stability . In addition ,good permeability was observed for both . The large specific surface area and good permeability of ZrO2/SiO2 permitted a high loading of chiral polymer Onto it , and greatly improved the enantioselectivity and the resolution for chiral separations E.Guihen,TrendsAnal.Chem.46(2013)1-14 15
  • 16. Gas chromatographyGas chromatography • GC has seen even less activity in relation to the use of NP stationary phases. Perhaps this may be because GC has enjoyed very high efficiency due to the long capillary Lengths and fast diffusion rates compared to LC. Also , the stationary phases in GC need to be very inert and withstand very high temperatures , so they may not be suitable for NPs E.Guihen,TrendsAnal.Chem.46(2013)1-14 16
  • 17. Gas chromatographyGas chromatography • Na et al developed a ionic liquid-dispersed silica NPs(IL-SN)capillary column, Combining properties of silica NPs and IL for GC . By Dispersing silica NPs in a conventional ILof1-butyl-3-Methyl imidazolium hexafluoro phosphate([Bu- MIm][BF6]) This coating integrated the advantages of silica NPs (i.e. large surface area ,and highly dispersed behavior)and the IL(i.e. extended liquid State temperature range ,and chemical stability),hence increasing interactions between the stationary phase and target analytes . Mixtures of a wide range of organic compounds ,Including alcohols , esters , alkanes , aromatic compounds, as well as isomers and non-polar compounds ,were Separated using the IL-SN capillary column . E.Guihen,TrendsAnal.Chem.46(2013)1-14 17
  • 18. Gas chromatographyGas chromatography E.Guihen,TrendsAnal.Chem.46(2013)1-14 18 GC chromatograms of mixtures of volatile(A)and non-volatile (B)aromatic compounds by three capillary columns .(a)Ionic liquid-dispersed silica nanoparticles (NPs)capillary column;(b)silver NPs capillary column ;and,(c)ionic liquid capillary column .Column temperature Was 50 C for(A),and 50 C (5min)–100 C (20min)for(B)
  • 19. MicrochipMicrochip electrophoresiselectrophoresis E.Guihen,TrendsAnal.Chem.46(2013)1-14 19 For protein, DNA, RNA and other important bio molecular analysisFor protein, DNA, RNA and other important bio molecular analysis Where by pre concentration ,derivatization ,separation and detection of complex biological samples are carried out on-chip based plat forms
  • 20. SeparationSeparation mechanismmechanism of the electrophoreticof the electrophoretic microchipmicrochip systemsystem E.Guihen,TrendsAnal.Chem.46(2013)1-14 20
  • 21. Microchip electrophoresisMicrochip electrophoresis Liang et al developed for the modification of a poly (dimethylsiloxane) (PDMS) microchip channel with polydopamine/AuNPs(PDA/AuNPs) •The AuNPs were highly and uniformly dispersed in/on the polydopamine matrix with an arrow size distribution . A high-speed , efficient separation of five amino Acid was obtained on the poly dopamine / AuNP-functionalized PDMS microchips. E.Guihen,TrendsAnal.Chem.46(2013)1-14 21
  • 22. Types of Columns E.Guihen,TrendsAnal.Chem.46(2013)1-14 22
  • 23. Capillary Electrophoresis And Open Tubular capillary electro chromatography • NPs use in OTCEC as inner wall coatings or powerful additives to the background electrolyte(or used as pseudo stationary phase(PSP)-CEC where by NPs are added to the buffer) • highly efficient electro chromatographic separations by using alkylthiol Au NPs • Nilsson et al. High lighted that NP-based CEC gives high speed and resolution , greater peak capacity ,requires small amounts of sample and can be coupled with MS • Zhu et al coated the inner walls of a capillary for OTCEC with magnetic NPs E.Guihen,TrendsAnal.Chem.46(2013)1-14 23
  • 24. Capillary Electrophoresis And Open Tubular capillary electro chromatography • Yang et al used cyclodextrin-modified AuNPs for enantiomeric separation of drugs and amino acids E.Guihen,TrendsAnal.Chem.46(2013)1-14 24
  • 25. Capillary Electrophoresis And Open Tubular capillary electro chromatography • Qu et al investigated the use of an octadecyl-amine capped AuNP capillary column for bio analysis • Wang et al Added silica NPs to the buffer in CE • Li et al demonstrated the use of amphiphilic silica NPs as PSP in CE for separation of basic and aromatic compounds E.Guihen,TrendsAnal.Chem.46(2013)1-14 25
  • 26. Ion chromatographyIon chromatography Monolithic columns are frequently used in separation science Cao and co-workers developed a porous polymer monolithic capillary column modified with Au NPs coated with exchange able functionalities that allowed easy switching of separation modes by a simple ligand-exchange process E.Guihen,TrendsAnal.Chem.46(2013)1-14 26
  • 27. Ion chromatographyIon chromatography Surface modifications of a single poly (glycidyl methacrylate-co-ethylene dimethacrylate)Surface modifications of a single poly (glycidyl methacrylate-co-ethylene dimethacrylate) monolith reacted with cystamine and containing gold nanoparticles (AuNPs)monolith reacted with cystamine and containing gold nanoparticles (AuNPs) The dashed arrow indicates that the same functionality can also be prepared via direct reaction of the AuNPs containing the monolith with the respective thiol group containing compound E.Guihen,TrendsAnal.Chem.46(2013)1-14 27 First, the column is treated with3-thio propionic acid ,then with mercaptoethanol ,and finally with cystamine .
  • 28. Ion chromatographyIon chromatography E.Guihen,TrendsAnal.Chem.46(2013)1-14 28 Separation of proteins in ion exchange mode using a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic column with attached gold nanoparticles (Au NPs) and modified with sodium 2-mercapto ethane sulfonate . (1)myoglobin;(2)ribonuclease 3) cytochrome
  • 29. 29

Related Documents