Thermostable DNA polymerasefrom thermophiles found in localhot springs: Expression andcharacterization By Mohd Khairul Fad...
 Selected 20 colonies Dilution streaking to get single colony Grow in nutrient broths 60°C Grow in nutrient br...
Gram stain
 Sense primer 5’-GAT-CCG-AAC-CTG-CAA-AAC-ATT-CC- 3’ Antisense primer 5’-CAG-GTG-CAT-GAC-GAG-CTG-ATT-3’ ...
Polymerase presentation 2
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Polymerase presentation 2

Published on: Mar 4, 2016
Source: www.slideshare.net


Transcripts - Polymerase presentation 2

  • 1. Thermostable DNA polymerasefrom thermophiles found in localhot springs: Expression andcharacterization By Mohd Khairul Fadzly HaronSupervised by: Prof. Dr. Raja Noor Zaliha Raja Abd. Rahman
  • 2.  Selected 20 colonies Dilution streaking to get single colony Grow in nutrient broths 60°C Grow in nutrient broths 80°C (2 days) Spread on plates (nutrient agar) Dilution streaking Gram staining
  • 3. Gram stain
  • 4.  Sense primer 5’-GAT-CCG-AAC-CTG-CAA-AAC-ATT-CC- 3’ Antisense primer 5’-CAG-GTG-CAT-GAC-GAG-CTG-ATT-3’ (Akhmaloka et al.,2008)

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