NANOBIOTEC Multiplex Assay For Cytokine
Production From PBMC
CONFIDENTIAL
http://www.nanobiotecusa.com/
© NANOBIOTEC, 2012
NO More ELISA, Use Luminex Assay
VEGF, sCD40L, EGF, Eotaxin, FGF-2, Flt-
3 ligand, Fractalkine, G-CSF, GM-CSF,
GRO, IFN-α2...
unstimulated
LPS stimulated
LPS + Cmpd1
LPS + Cmpd2
LPS + Cmpd3
LPS + Cmpd4
LPS + Cmpd5
LPS + Cmpd6
Effects of Compounds o...
Cytokines Significantly Inhibited by Compounds
**
**
0 .1
1
1 0
1 0 0
1 0 0 0
1 0 0 0 0
1 0 0 0 0 0
G M C S F IL 1 2 p 4 0...
Effect of compound on cytokine levels of patient PBMC
Production of many cytokines can be
stimulated in PBMCs with LPS , a...
Bioinformatic analysis for multiplex data
Our team has unparalleled computational
expertise and our employees were trained...
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Nanobiotec luminex assay

Nanobiotec luminex assay service data example
Published on: Mar 3, 2016
Published in: Health & Medicine      
Source: www.slideshare.net


Transcripts - Nanobiotec luminex assay

  • 1. NANOBIOTEC Multiplex Assay For Cytokine Production From PBMC CONFIDENTIAL http://www.nanobiotecusa.com/ © NANOBIOTEC, 2012
  • 2. NO More ELISA, Use Luminex Assay VEGF, sCD40L, EGF, Eotaxin, FGF-2, Flt- 3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNF-α, TNF-β We measure 2~41-plex cytokines/chemokines in 100 ml samples (cell culture supernatants, serum, etc.) to save time and money
  • 3. unstimulated LPS stimulated LPS + Cmpd1 LPS + Cmpd2 LPS + Cmpd3 LPS + Cmpd4 LPS + Cmpd5 LPS + Cmpd6 Effects of Compounds on 25 Cytokines from PBMC
  • 4. Cytokines Significantly Inhibited by Compounds ** ** 0 .1 1 1 0 1 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 G M C S F IL 1 2 p 4 0IL 1  IP 1 0 M C P -1M IP 1  T N F IF N  b lu e : u n s tim u la te d re d : s tim u la te d , D M S O g re e n : s tim u la te d , N B T -0 4 0 b ro w n : s tim u la te d , N B T -0 5 0 pg/ml t-te s t, p a ire d * p < 0 .0 5 , ** p < 0 .0 1 , *** p < 0 .0 0 1 ** ** *** *** *** *** ns ns *** *** *** *** *** ***
  • 5. Effect of compound on cytokine levels of patient PBMC Production of many cytokines can be stimulated in PBMCs with LPS , and NBT-069 blocks the production when treated cells for 24 h. cytokine levels from patient PBMC+ LPS+ NBTs Number = cytokine levels from patient PBMC +LPS+ DMSO LPS LPS + 100 nM NBT-069 DMSO NBT-010 NBT-020 NBT-030 NBT-040 NBT-050
  • 6. Bioinformatic analysis for multiplex data Our team has unparalleled computational expertise and our employees were trained at MIT and NIH, and worked over a decade for multinational biopharma companies in NJ, USA. We can help interpret the data from multiple experiments to address the potential mechanism of actions of test compounds.

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