Nathan H. McNeill, M.S.
9039 Redondo Dr.
Dallas, Tx. 75218
972 345-2208
Personal email: nathanhmcneill@gmail.com
Place of ...
RT-PCR, RFLP, vector design, DNA/RNA/protein/lymphocyte isolation,
confocal/florescence microscopy, immunocytochemistry, g...
 Mouse cardiomyocyte enrichment and primary culturing of mouse
embryonic fibroblasts
o Utilized Real-time PCR analysis, R...
• Baylor University Scholarship and Stipend for Biomedical Sciences Doctoral
Program
• Golden Key Honor Society
• Academic...
• Cantarel BL, Weaver D, McNeill N, Zhang J, Mackey AJ, Reese J. BAYSIC: a
Bayesian method for combining sets of genome va...
• Cantarel BL, Weaver D, McNeill N, Zhang J, Mackey AJ, Reese J. BAYSIC: a
Bayesian method for combining sets of genome va...
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Nathan McNeill CV Complete

Published on: Mar 3, 2016
Source: www.slideshare.net


Transcripts - Nathan McNeill CV Complete

  • 1. Nathan H. McNeill, M.S. 9039 Redondo Dr. Dallas, Tx. 75218 972 345-2208 Personal email: nathanhmcneill@gmail.com Place of Birth: Dallas, Texas Citizenship: United States I am a motivated doctoral candidate with a targeted spring 2016 date for Ph.D completion in biomedical sciences from Baylor University. My passion for learning, problem solving, and discovery has shaped me into a focused, results-oriented critical and analytical thinker. I thrive in both collaborative and independent working environments and I am well-versed in both clinical and basic research processes, regulatory standards for clinical diagnostics, managing and teaching students and research assistants, and managing, running, and maintaining a molecular diagnostics laboratory. I am experienced in multiple scientific fields including genetics, genomics, and molecular and cell biology spanning multiple disease states including leukoencephalopathies and other neurological disorders, inborn errors of metabolism, and cardiovascular disease. Professional Skills: • Background includes Molecular Genetics, Genomics, Biotechnology, and Molecular and Cell Biology with knowledge of a wide array of biotechnologies and experience in a wide range of experimental techniques and methods • Experience in Clinical Molecular Genetic Diagnostics and Clinical/Basic Research and Development in the areas of rare inborn errors of metabolism, cardiovascular disease, and Leukoencephalopathies • Experience in managing and maintaining a Clinical Molecular Diagnostics laboratory including overseeing daily lab operations, quality assurance, equipment maintenance, and developing and implementing novel genetic testing and supervising technicians and graduate students • Bioinformatics experience includes but not limited to: next generation sequence data analysis, BLAST and multiple sequence alignment analysis, primer/mutagenesis design, splicing and RNA secondary structure analysis, proficient use in genome browsers and population databases, and LIMS Additional Skills: • Experience in the following: sanger sequencing, next generation whole exome sequencing, bioinformatic analysis of next generation sequencing data, gene microarray, primer design, human and mouse iPS cell/cardiomyocyte/fibroblast cell culture, cloning, site-directed mutagenesis, western blot, real time PCR, PCR,
  • 2. RT-PCR, RFLP, vector design, DNA/RNA/protein/lymphocyte isolation, confocal/florescence microscopy, immunocytochemistry, gel electrophoresis, ELISA, mutation analysis, morpholino design, in situ hybridization, in vivo modeling and functional testing in zebrafish • Programming skills include use of command line and Unix programming and other computer skills include Microsoft Word, Excel, and Powerpoint • Excellent planning and organizational skills with the ability to multi-task and prioritize • Strong collaborative and interpersonal skills • Drafting and Illustration skills Education: • 2000: High School Valedictorian graduate from Canyon Creek Christian Academy, Richardson, TX. • 2004: B.S. in Biology at the University of Texas at Dallas • 2006: M.S. in Biotechnology at the University of Texas at Dallas • 2016 (Pending): PhD in Biomedical Sciences at Baylor University o Mentor: Dr. Raphael Schiffmann o Dissertation Title: “Uncovering the Genetic Etiologies of Unsolved Leukodystrophies Using Next Generation Whole Exome Sequencing” Employment History: • 2010-Present: PhD candidate in Biomedical Sciences at Baylor University o Dissertation research aims to identify the underlying genetic etiologies of unsolved leukodystrophies using next generation whole exome sequencing and to functionally validate the causal variants o Many different bioinformatics tools and databases used in next generation sequence analysis o Functional validation of causal variants carried out by in-silico analysis, in-vitro experimentation, and in-vivo zebrafish assays o Research was carried out at Children’s National Medical Center in Washington D.C. under Dr. Eric Hoffman and Dr. Adeline Vanderver, NIH NHGRI in Bethesda, Maryland, the Center for Human Disease Modeling at Duke University under the direction of Dr. Nicolas Katsanis and Dr. Erica Davis, and Dallas Baylor Scott and White under Dr. Raphael Schiffmann • 2009-2010: Research assistant at Institute of Metabolic Disease at Baylor Research Institute, Dallas, Texas o Performed cell culture in the generation, maintenance, and differentiation of human induced pluripotent stem (iPS) cells and in the study of the pathogenesis of Fabry disease using mouse iPS cells
  • 3.  Mouse cardiomyocyte enrichment and primary culturing of mouse embryonic fibroblasts o Utilized Real-time PCR analysis, RNA isolation, cDNA generation, and biomarker staining • 2006-2010: Metabolic Technologist at the Institute of Metabolic Disease at Baylor University Medical Center o Performed molecular diagnostics and clinical diagnostic research in a CAP accredited molecular genetics diagnostics laboratory o Performed diagnostics for fatty acid oxidation/metabolic disorders, cardiovascular disease, and neurological disorders including MCADD, LCADD, VLCADD, SCADD, CPTII deficiency, Fabry disease, Wolff- Parkinson-White Syndrome, Leukoencephalopathy With Vanishing White Matter, Alpha-1-Antitrypsin Deficiency, and Diabetes o Recorded patient data, designed SOPs, performed quality control assays o No deficiencies in regulatory inspections by CAP o Maintained records ad upheld lab practices in accordance with CAP, CLIA, FDA, and HIPAA regulatory guidelines o Responsibilities included management of laboratory activities, research assistants, and graduate students and day to day running and maintenance of the laboratory including equipment and inventory o Duties utilized techniques including DNA and RNA isolation, PCR, RFLP analysis, cloning, and sequencing for mutation analysis in clinical patients o Performed enzyme assays utilizing radioactive isotopes from fibroblasts and isolated lymphocytes o Development of new Molecular Diagnostic assays for heart disease, neurological disorders, and inborn errors of metabolism o All sequencing work done on ABI 3100 and 310 Genetic Analyzers o Discovered several novel mutations in the medium chain acyl-CoA dehydrogenase (MCAD) gene and the carnitine palmitoyl transferase 2 (CPT-II) gene • 2005-2006: Research Technician at the Institute of Metabolic Disease at Baylor o Worked on developing new clinical diagnostic testing methods for the inborn error of metabolism medium chain acyl-CoA dehydrogenase deficiency (MCADD) using DNA microarray o DNA primer testing and cloning experiments on genes involved with diabetes and hypertrophic cardiomyopathy o Daily lab maintenance and quality control for lab equipment Honors/Awards:
  • 4. • Baylor University Scholarship and Stipend for Biomedical Sciences Doctoral Program • Golden Key Honor Society • Academic Distinction Scholarship to the University of Texas at Dallas • State of Texas Valedictorian Scholarship • High School Valedictorian • Who’s Who Among American High School Students Volunteer Work: • Held position of publicity chair for the Engineering in Medicine and Biology Society (EMBS) workshop of the Institute of Electrical and Electronic Engineers’ (IEEE) Dallas chapter • Worked at several events conducted by the Dallas-Ft. Worth Asian American Citizen’s Council (AACC) • Helped raise funds in the 2008 campaign of Texas state representative Angie Button • Helped prepare and organize 2013 Leukodystrophy Consortium • Volunteer for the BioNorthTx inaugural breaksfast at Baylor Scott&White Sammon’s Cancer Center Publications/abstracts: • Medium-chain acyl-CoA dehydrogenase deficiency-the molecular aspect. The American Society of Human Genetics 59th Annual Meeting, 2009. • The molecular aspect of carnitine palmitoyl transferase 2(CPT-II) deficiency. The American Society of Human Genetics 59th Annual Meeting, 2009; • Novel Mutations in Carnitine palmitoyltransferase II (CPT-II) gene. The American Society of Human Genetics 57th Annual Meeting 2007; • Novel Mutations in medium chain acyl-CoA dehydrogenase gene. The American Society of Human Genetics 56th Annual Meeting 2006. • Mochel F, Yang B, Barritault J, Thompson JN, Engelke UF, McNeill NH, Benko WS, Kaneski CR, Adams DR, Tsokos M, Abu-Asab M, Huizing M, Seguin F, Wevers RA, Ding J, Verheijen FW, Schiffmann R. Free sialic acid storage disease without sialuria. Ann Neurol. 2009 Jun;65(6):753-7. • Mochel F, Engelke UF, Barritault J, Yang B, McNeill NH, Thompson JN, Vanderver A, Wolf NI, Willemsen MA, Verheijen FW, Seguin F, Wevers RA, Schiffmann R. Elevated CSF N-acetylaspartylglutamate in patients with free sialic acid storage diseases. Neurology. 2010 Jan 26;74(4):302-5. • Parikh S, Bernard G, Leventer RJ, van der Knaap MS, van Hove J, Pizzino A, McNeill NH, Helman G, Simons C, Schmidt JL, et al. A clinical approach to the diagnosis of patients with leukodystrophies and genetic leukoencephelopathies. Mol Genet Metab. 2015 Apr;114(4):501-15.
  • 5. • Cantarel BL, Weaver D, McNeill N, Zhang J, Mackey AJ, Reese J. BAYSIC: a Bayesian method for combining sets of genome variants with improved specificity and sensitivity. BMC Bioinformatics. 2014 Apr 12;15:104. • Simons C, Wolf NI, McNeil N, Caldovic L, Devaney JM, Takanohashi A, Crawford J, Ru K, Grimmond SM, Miller D, Tonduti D, Schmidt JL, Chudnow RS, van Coster R, Lagae L, Kisler J, Sperner J, van der Knaap MS, Schiffmann R, Taft RJ, Vanderver A. A de novo mutation in the β-tubulin gene TUBB4A results in the leukoencephalopathy hypomyelination with atrophy of the basal ganglia and cerebellum. Am J Hum Genet. 2013 May 2;92(5):767-73. • Vanderver A, et al. Whole exome sequencing in patients with central nervous system white matter abnormalities. (Pending) • Shen, Jin-Song, et al. Molecular basis for globotriaosylceramide regulation and enzyme uptake in immortalized aortic endothelial cells from Fabry mice. (Pending)
  • 6. • Cantarel BL, Weaver D, McNeill N, Zhang J, Mackey AJ, Reese J. BAYSIC: a Bayesian method for combining sets of genome variants with improved specificity and sensitivity. BMC Bioinformatics. 2014 Apr 12;15:104. • Simons C, Wolf NI, McNeil N, Caldovic L, Devaney JM, Takanohashi A, Crawford J, Ru K, Grimmond SM, Miller D, Tonduti D, Schmidt JL, Chudnow RS, van Coster R, Lagae L, Kisler J, Sperner J, van der Knaap MS, Schiffmann R, Taft RJ, Vanderver A. A de novo mutation in the β-tubulin gene TUBB4A results in the leukoencephalopathy hypomyelination with atrophy of the basal ganglia and cerebellum. Am J Hum Genet. 2013 May 2;92(5):767-73. • Vanderver A, et al. Whole exome sequencing in patients with central nervous system white matter abnormalities. (Pending) • Shen, Jin-Song, et al. Molecular basis for globotriaosylceramide regulation and enzyme uptake in immortalized aortic endothelial cells from Fabry mice. (Pending)

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