nanosyn.com
DISCOVERY
DELIVERED
Accelerating your drug discoveryOlga Issakova
Executive Vice President
(408) 987-2004
oiss...
Accelerating your drug discovery
Nanosyn is a biological and chemical services contract research
organization providing hi...
Accelerating your drug discovery
nanosyn.com
In contrast to other high throughput screening
fluorescence- and luminescence...
nanosyn.com
Accelerating your drug discovery
ABL1 ABL1 c-abl oncogene 1, non-receptor tyrosine kinase
AKT1 AKT1 v-akt muri...
nanosyn.com
Accelerating your drug discovery
CLK4 CLK4 CDC-like kinase 4
CRAF RAF1 v-raf-1 murine leukemia viral oncogene ...
nanosyn.com
Accelerating your drug discovery
HIPK4 HIPK4 homeodomain interacting protein kinase 4
IGF1R IGF1R insulin-like...
nanosyn.com
Accelerating your drug discovery
MUSK MUSK muscle, skeletal, receptor tyrosine kinase
NDR2 STK38L serine/threo...
nanosyn.com
Accelerating your drug discovery
PRKD1 PRKD1 protein kinase D1
PRKD2 PRKD2 protein kinase D2
PRKD3 PRKD3 prote...
nanosyn.com
Accelerating your drug discovery
HDAC 1 HDAC1 histone deacetylase 1 Deacetylases/SIRT
HDAC 2 HDAC2 histone dea...
nanosyn.com
Accelerating your drug discovery
PDE1A1 PDE1A1 phosphodiesterase 1A, calmodulin-dependent
PDE1B  PDE1B  phosp...
CD45 PTPRC protein tyrosine phosphatase, receptor type, C
LAR PTPRF protein tyrosine phosphatase, receptor type, F
PTP-Bet...
Aspartic BACE1 beta-site APP-cleaving enzyme 1
Aspartic CTSD CATHEPSIN D
Cysteine CAPN1 Calpain-1
Cysteine CASP1 CASPASE 1...
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Nanosyn Profiling Overview - Full

Published on: Mar 3, 2016
Source: www.slideshare.net


Transcripts - Nanosyn Profiling Overview - Full

  • 1. nanosyn.com DISCOVERY DELIVERED Accelerating your drug discoveryOlga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 2. Accelerating your drug discovery Nanosyn is a biological and chemical services contract research organization providing high throughput screening, compound profiling, assay development, protein expression, SAR expansion and testing, medicinal chemistry, compound libraries, hit-to-lead optimization, process chemistry, scale-up services and other innovative solutions to meet your drug discovery needs. Our comprehensive platform of kinase, protease, phosphatase and epigenetic assays utilizes cutting- edge microfluidics and informatics tools to provide accurate and rapid results for your drug discovery, compound evaluation and prioritization programs. SCREENING AND PROFILING SERVICESNanosyn’s biochemical assays utilize a Caliper LabChip microfluidic technology—the only profiling technology that affords simultaneous detection and quantification of both substrate and product of the reaction in the same sample. The internal normalization of product to substrate reduces the effects of sampling errors and results in highly precise measurements. The separation of product from substrate also minimizes effect of fluorescent compounds on the assay readout. The robustness of our assays is documented by Z’ values higher than 0.75. The intrinsically low background values also allow for a high sensitivity of detection with minimal amounts of substrate, enzyme and compound required. To ensure the quality we utilize only the highest quality proteins that we source from the most reliable commercial vendors or produce in house. MICROFLUIDICS-BASED DETECTION TECHNOLOGY Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 3. Accelerating your drug discovery nanosyn.com In contrast to other high throughput screening fluorescence- and luminescence-based assays, including Alpha-Screen, IMAP and Fluor de Lys, the mobility shift assay tolerates significant levels of auto- fluorescence associated with many chemical compounds, allowing unambiguous detection of positive compounds. An in-house set of compounds clearly shows that the signature of auto-fluorescent compounds was unmistakably different from that of a positive inhibitor/activator trace. In addition, the simultaneous detection of product and substrate provides means for internal normalization of individual enzymatic reactions, resulting in significantly improved reproducibility. THE PRINCIPLE OF MICROFLUIDIC DETECTION Enzymatic reactions are performed in 384-well microplates and the 12-channel Caliper LabChip instrument is used as a detection device. The enzymatic modification of a peptide results in a change in net charge, enabling electrophoretic separation of product from substrate. As substrate and product are separated, two peaks of fluorescence are observed. Change in the relative fluorescence intensity of the substrate and product peaks is the parameter measured, reflecting enzyme activity. In the presence of an inhibitor, the ratio between product and substrate is altered: the signal of the product decreases, while the signal of the substrate increases. Direct readout of enzyme activity by monitoring product and substrate in the same run Easily identify fluorescent compounds interfering with assays High precision and reproducibility of the data Even weak inhibitors can be readily identified Low level of false positive and false negatives Applicable to a wide range of targets Activators as well as inhibitors readily identified in a single screen ADVANTAGES Enzyme Labeled substrate 384 well plate w/compounds P S S Ac K K Kinases or Phosphatases HATs or HDACs Peptidases product substrate compound Enzyme Labeled substrate 384 well plate w/compounds P S S P S P SS SSS Ac K K Ac K Ac KK KKKK Kinases or Phosphatases HATs or HDACs Peptidases product substrate product substrate compoundcompound Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 4. nanosyn.com Accelerating your drug discovery ABL1 ABL1 c-abl oncogene 1, non-receptor tyrosine kinase AKT1 AKT1 v-akt murine thymoma viral oncogene homolog 1 AKT2 AKT2 v-akt murine thymoma viral oncogene homolog 2 AKT3 AKT3 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) ALK ALK anaplastic lymphoma receptor tyrosine kinase AMP-A1B1G1 PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit AMP-A2B1G1 PRKAA2 protein kinase, AMP-activated, alpha 2 catalytic subunit ARG ABL2 v-abl Abelson murine leukemia viral oncogene homolog 2 ARK5 NUAK1 NUAK family, SNF1-like kinase, 1 AURORA-A AURKA aurora kinase A AURORA-B AURKB aurora kinase B AURORA-C AURKC aurora kinase C AXL AXL AXL receptor tyrosine kinase BLK BLK B lymphoid tyrosine kinase BMX BMX BMX non-receptor tyrosine kinase BRAF BRAF v-raf murine sarcoma viral oncogene homolog B1 BRK PTK6 PTK6 protein tyrosine kinase 6 BRSK1 BRSK1 BR serine/threonine kinase 1 BRSK2 BRSK2 BR serine/threonine kinase 2 BTK BTK Bruton agammaglobulinemia tyrosine kinase CAMK1A CAMK1 calcium/calmodulin-dependent protein kinase I CAMK1D CAMK1D calcium/calmodulin-dependent protein kinase ID CAMK2A CAMK2A calcium/calmodulin-dependent protein kinase II alpha CAMK2B CAMK2B calcium/calmodulin-dependent protein kinase II beta CAMK2G CAMK2G calcium/calmodulin-dependent protein kinase II gamma CAMK2D CAMK2D calcium/calmodulin-dependent protein kinase II delta CAMK4 CAMK4 calcium/calmodulin-dependent protein kinase IV CDK1-CYCLINB CDK1 cyclin-dependent kinase 1 / cyclinB CDK2-CYCLINA CDK2 cyclin-dependent kinase 2 / cyclinA CDK2-CYCLINE CDK2 cyclin-dependent kinase 2 / cyclinE CDK3-CYCLINE1 CDK3 cyclin-dependent kinase 3 / cyclinE1 CDK4-CYCLIND1 CDK4 cyclin-dependent kinase 4 / cyclinD1 CDK5-p35 CDK5 cyclin-dependent kinase 5 / p35 CDK5-p25 CDK5 cyclin-dependent kinase 5 / p25 CDK6-CYCLIND3 CDK6 cyclin-dependent kinase 6 / cyclinD3 CDK7/CICLINE H CDK7 cyclin-dependent kinase 7/cyclin H CDK9/CYCLINE T1 CDK9 cyclin-dependent kinase 9 / cyclin T1 CHEK1 CHEK1 CHK1 checkpoint human homolog (S. pombe) CHEK2 CHEK2 CHK2 checkpoint human homolog (S. pombe) CK1-ALPHA1 CSNK1A1 casein kinase 1, alpha 1 CK1-EPSILON CSNK1E casein kinase 1, epsilon CK1-GAMMA1 CSNK1G1 casein kinase 1, gamma 1 CK1-GAMMA2 CSNK1G2 casein kinase 1, gamma 2 CK1-GAMMA3 CSNK1G3 casein kinase 1, gamma 3 CK2 CSNK2A1 casein kinase 2, alpha 1 polypeptide CLK1 CLK1 CDC-like kinase 1 CLK2 CLK2 CDC-like kinase 2 CLK3 CLK3 CDC-like kinase 3 Available Kinase Assays Nanosyn Name Symbol Approved Name Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 5. nanosyn.com Accelerating your drug discovery CLK4 CLK4 CDC-like kinase 4 CRAF RAF1 v-raf-1 murine leukemia viral oncogene homolog 1 CSK CSK c-src tyrosine kinase DAPK1 DAPK1 death-associated protein kinase 1 DAPK3 DAPK3 death-associated protein kinase 3 DCAMKL2 DCLK2 doublecortin-like kinase 2 DDR1 DDR1 discoidin domain receptor tyrosine kinase 1 DDR2 DDR2 discoidin domain receptor tyrosine kinase 2 DYRK1A DYRK1A dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A DYRK1B DYRK1B dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B DYRK2 DYRK2 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 DYRK3 DYRK3 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 DYRK4 DYRK4 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 EGFR EGFR epidermal growth factor receptor EPH-A1 EPHA1 EPH receptor A1 EPH-A2 EPHA2 EPH receptor A2 EPH-A3 EPHA3 EPH receptor A3 EPH-A4 EPHA4 EPH receptor A4 EPH-A5 EPHA5 EPH receptor A5 EPH-A8 EPHA8 EPH receptor A8 EPH-B1 EPHB1 EPH receptor B1 EPH-B2 EPHB2 EPH receptor B2 EPH-B3 EPHB3 EPH receptor B3 EPH-B4 EPHB4 EPH receptor B4 ERBB2/HER2 ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog ERBB4/HER4 ERBB4 v-erb-a erythroblastic leukemia viral oncogene homolog 4 FAK PTK2 PTK2 protein tyrosine kinase 2 FER FER fer (fps/fes related) tyrosine kinase FES FES feline sarcoma oncogene FGFR1 FGFR1 fibroblast growth factor receptor 1 FGFR2 FGFR2 fibroblast growth factor receptor 2 FGFR3 FGFR3 fibroblast growth factor receptor 3 FGFR4 FGFR4 fibroblast growth factor receptor 4 FGR FGR Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog FLT1 FLT1 fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) FLT3 FLT3 fms-related tyrosine kinase 3 FLT4 FLT4 fms-related tyrosine kinase 4 FMS CSF1R colony stimulating factor 1 receptor FRAP1 MTOR mechanistic target of rapamycin (serine/threonine kinase) FYN FYN FYN oncogene related to SRC, FGR, YES GRK6 GRK6 G protein-coupled receptor kinase 6 GRK7 GRK7 G protein-coupled receptor kinase 7 GSK3-ALPHA GSK3A glycogen synthase kinase 3 alpha GSK3-BETA GSK3B glycogen synthase kinase 3 beta HASPIN GSG2 germ cell associated 2 (haspin) HCK HCK hemopoietic cell kinase HIPK1 HIPK1 homeodomain interacting protein kinase 1 HIPK2 HIPK2 homeodomain interacting protein kinase 2 HIPK3 HIPK3 homeodomain interacting protein kinase 3 Nanosyn Name Symbol Approved Name Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 6. nanosyn.com Accelerating your drug discovery HIPK4 HIPK4 homeodomain interacting protein kinase 4 IGF1R IGF1R insulin-like growth factor 1 receptor IKK-ALPHA CHUK conserved helix-loop-helix ubiquitous kinase IKK-BETA IKBKB inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta IKK-EPSILON IKBKE inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon INSR INSR insulin receptor IRAK1 IRAK1 interleukin-1 receptor-associated kinase 1 IRAK4 IRAK4 interleukin-1 receptor-associated kinase 4 IRR INSRR insulin receptor-related receptor ITK ITK IL2-inducible T-cell kinase JAK1 JAK1 Janus kinase 1 JAK2 JAK2 Janus kinase 2 JAK3 JAK3 Janus kinase 3 JNK1 MAPK8 mitogen-activated protein kinase 8 JNK2 MAPK9 mitogen-activated protein kinase 9 JNK3 MAPK10 mitogen-activated protein kinase 10 KDR KDR kinase insert domain receptor (a type III receptor tyrosine kinase) KIT KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog LATS1 LATS1 large tumor suppressor kinase 1 LATS2 LATS2 LATS, large tumor suppressor, homolog 2 (Drosophila) LCK LCK lymphocyte-specific protein tyrosine kinase LOK STK10 serine/threonine kinase 10 LRRK2 LRRK2 leucine-rich repeat kinase 2 LTK LTK leukocyte receptor tyrosine kinase LYNA LYN v-yes-1 Yamaguchi sarcoma viral related oncogene homolog, isoform A LYNB LYN v-yes-1 Yamaguchi sarcoma viral related oncogene homolog, isoform B MAP4K2 MAP4K2 mitogen-activated protein kinase kinase kinase kinase 2 MAP4K4 MAP4K4 mitogen-activated protein kinase kinase kinase kinase 4 MAP4K5 MAP4K5 mitogen-activated protein kinase kinase kinase kinase 5 MAPK1 MAPK1 mitogen-activated protein kinase 1 MAPK3 MAPK3 mitogen-activated protein kinase 3 MAPKAPK2 MAPKAPK2 mitogen-activated protein kinase-activated protein kinase 2 MAPKAPK3 MAPKAPK3 mitogen-activated protein kinase-activated protein kinase 3 MARK1 MARK1 MAP/microtubule affinity-regulating kinase 1 MARK3 MARK3 MAP/microtubule affinity-regulating kinase 3 MARK4 MARK4 MAP/microtubule affinity-regulating kinase 4 MEK1 MAP2K1 mitogen-activated protein kinase kinase 1 MEK2 MAP2K2 mitogen-activated protein kinase kinase 2 MELK MELK maternal embryonic leucine zipper kinase MER MERTK c-mer proto-oncogene tyrosine kinase MET MET met proto-oncogene (hepatocyte growth factor receptor) MINK MINK1 misshapen-like kinase 1 MKNK1 MKNK1 MAP kinase interacting serine/threonine kinase 1 MRCK-ALPHA CDC42BPA CDC42 binding protein kinase alpha (DMPK-like) MRCK-BETA CDC42BPB CDC42 binding protein kinase beta (DMPK-like) MSK1 RPS6KA5 ribosomal protein S6 kinase, 90kDa, polypeptide 5 MSK2 RPS6KA4 ribosomal protein S6 kinase, 90kDa, polypeptide 4 MSSK1 SRPK3 SRSF protein kinase 3 MST1 STK4 serine/threonine kinase 4 MST2 STK3 serine/threonine kinase 3 MST3 STK24 serine/threonine kinase 24 Nanosyn Name Symbol Approved Name Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 7. nanosyn.com Accelerating your drug discovery MUSK MUSK muscle, skeletal, receptor tyrosine kinase NDR2 STK38L serine/threonine kinase 38 like NDRG1 NDRG1 N-myc downstream regulated 1 NEK1 NEK1 NIMA (never in mitosis gene a)-related kinase 1 NEK2 NEK2 NIMA (never in mitosis gene a)-related kinase 2 NEK6 NEK6 NIMA (never in mitosis gene a)-related kinase 6 NEK7 NEK7 NIMA (never in mitosis gene a)-related kinase 7 NEK9 NEK9 NIMA (never in mitosis gene a)- related kinase 9 P38-ALPHA MAPK14 mitogen-activated protein kinase 14 P38-BETA MAPK11 mitogen-activated protein kinase 11 P38-DELTA MAPK13 mitogen-activated protein kinase 13 P38-GAMMA MAPK12 mitogen-activated protein kinase 12 P70S6K1 RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 P70S6K2 RPS6KB2 ribosomal protein S6 kinase, 70kDa, polypeptide 2 PAK1 PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 PAK2 PAK2 p21 protein (Cdc42/Rac)-activated kinase 2 PAK3 PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 PAK4 PAK4 p21 protein (Cdc42/Rac)-activated kinase 4 PAK5 PAK7 p21 protein (Cdc42/Rac)-activated kinase 7 PAK6 PAK6 p21 protein (Cdc42/Rac)-activated kinase 6 PAR-1B-ALPHA MARK2 MAP/microtubule affinity-regulating kinase 2 PASK PASK PAS domain containing serine/threonine kinase PDGFR-ALPHA PDGFRA platelet-derived growth factor receptor, alpha polypeptide PDGFR-BETA PDGFRB platelet-derived growth factor receptor, beta polypeptide PDK1 PDK1 pyruvate dehydrogenase kinase, isozyme 1 PERK EIF2AK3 eukaryotic translation initiation factor 2-alpha kinase 3 PHK-GAMMA1 PHKG1 phosphorylase kinase, gamma 1 (muscle) PHK-GAMMA2 PHKG2 phosphorylase kinase, gamma 2 (testis) PI3K-ALPHA PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide PI4K-BETA PI4KB phosphatidylinositol 4-kinase, catalytic, beta PIM1 PIM1 pim-1 oncogene PIM2 PIM2 pim-2 oncogene PIM3 PIM3 pim-3 oncogene PKA PRKACA protein kinase, cAMP-dependent, catalytic, alpha PKACB PRKACB protein kinase, cAMP-dependent, catalytic, beta PKC-ALPHA PRKCA protein kinase C, alpha PKC-BETA1 PRKCB protein kinase C, beta PKC-BETA2 PRKCB2 protein kinase C, beta PKC-ETA PRKCH protein kinase C, eta PKC-EPSILON PRKCE protein kinase C, epsilon PKC-GAMMA PRKCG protein kinase C, gamma PKC-IOTA PRKCI protein kinase C, iota PKC-THETA PRKCQ protein kinase C, theta PKC-ZETA PRKCZ protein kinase C, zeta PKN1 PKN1 protein kinase N1 PKN2 PKN2 protein kinase N2 PLK1 PLK1 polo-like kinase 1 PLK2 PLK2 polo-like kinase 2 PLK3 PLK3 polo-like kinase 3 PLK4 PLK4 polo-like kinase 4 PRAK MAPKAPK5 mitogen-activated protein kinase-activated protein kinase 5 Nanosyn Name Symbol Approved Name Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 8. nanosyn.com Accelerating your drug discovery PRKD1 PRKD1 protein kinase D1 PRKD2 PRKD2 protein kinase D2 PRKD3 PRKD3 protein kinase D3 PRKG1 PRKG1 protein kinase, cGMP-dependent, type I PRKG2 PRKG2 protein kinase, cGMP-dependent, type II PRKX PRKX protein kinase, X-linked PTK5 FRK fyn-related kinase PYK2 PTK2B PTK2B protein tyrosine kinase 2 beta RET RET ret proto-oncogene RIPK2 RIPK2 receptor-interacting serine-threonine kinase 2 ROCK1 ROCK1 Rho-associated, coiled-coil containing protein kinase 1 ROCK2 ROCK2 Rho-associated, coiled-coil containing protein kinase 2 RON MST1R macrophage stimulating 1 receptor (c-met-related tyrosine kinase) ROS ROS1 c-ros oncogene 1 , receptor tyrosine kinase RSK1 RPS6KA1 ribosomal protein S6 kinase, 90kDa, polypeptide 1 RSK2 RPS6KA3 ribosomal protein S6 kinase, 90kDa, polypeptide 3 RSK3 RPS6KA2 ribosomal protein S6 kinase, 90kDa, polypeptide 2 RSK4 RPS6KA6 ribosomal protein S6 kinase, 90kDa, polypeptide 6 SGK1 SGK1 serum/glucocorticoid regulated kinase 1 SGK2 SGK2 serum/glucocorticoid regulated kinase 2 SGK3 SGK3 serum/glucocorticoid regulated kinase family, member 3 SIK SIK1 salt-inducible kinase 1 SLK SLK STE20-like kinase SNF1LK2 SIK2 salt-inducible kinase 2 SPHK1 SPHK1 sphingosine kinase 1 SPHK2 SPHK2 sphingosine kinase 2 SRC SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog SRMS SRMS src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites SRPK1 SRPK1 SRSF protein kinase 1 SRPK2 SRPK2 SRSF protein kinase 2 STK16 STK16 serine/threonine kinase 16 SYK SYK spleen tyrosine kinase TAK1/TAB1 MAP3K7 mitogen-activated protein kinase kinase kinase 7 /TAB1 TAOK2 TAOK2 TAO kinase 2 TAOK3 TAOK3 TAO kinase 3 TBK1 TBK1 TANK-binding kinase 1 TEC TEC tec protein tyrosine kinase TIE2 TEK TEK tyrosine kinase, endothelial TNIK TNIK TRAF2 and NCK interacting kinase TNK2 TNK2 tyrosine kinase, non-receptor, 2 TRKA NTRK1 neurotrophic tyrosine kinase, receptor, type 1 TRKB NTRK2 neurotrophic tyrosine kinase, receptor, type 2 TRKC NTRK3 neurotrophic tyrosine kinase, receptor, type 3 TSSK1 TSSK1B testis-specific serine kinase 1B TSSK2 TSSK2 testis-specific serine kinase 2 TTK TTK TTK protein kinase TXK TXK TXK tyrosine kinase TYK2 TYK2 tyrosine kinase 2 TYRO3 TYRO3 TYRO3 protein tyrosine kinase YES YES1 v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 ZAP70 ZAP70 zeta-chain (TCR) associated protein kinase 70kDa Nanosyn Name Symbol Approved Name Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 9. nanosyn.com Accelerating your drug discovery HDAC 1 HDAC1 histone deacetylase 1 Deacetylases/SIRT HDAC 2 HDAC2 histone deacetylase 2 Deacetylases/SIRT HDAC 3 HDAC3 histone deacetylase 3 Deacetylases/SIRT HDAC 8 HDAC8 histone deacetylase 8 Deacetylases/SIRT HDAC 4 HDAC4 histone deacetylase 4 Deacetylases/SIRT HDAC 5 HDAC5 histone deacetylase 5 Deacetylases/SIRT HDAC 7 HDAC7 histone deacetylase 7 Deacetylases/SIRT HDAC 9 HDAC9 histone deacetylase 9 Deacetylases/SIRT HDAC 6 HDAC6 histone deacetylase 6 Deacetylases/SIRT HDAC 10 HDAC10 histone deacetylase 10 Deacetylases/SIRT HDAC11 HDAC11 histone deacetylase 11 Deacetylases/SIRT SIRT 1 SIRT1 sirtuin 1 Deacetylases/SIRT SIRT 2 SIRT2 sirtuin 2 Deacetylases/SIRT SIRT 3 SIRT3 sirtuin 3 Deacetylases/SIRT EP300 EP300 E1A binding protein p300 Acetyltransferases PCAF Acetyltransferases GCN5 Acetyltransferases G9a EHMT2 euchromatic histone-lysine N-methyltransferase 2 Methyltransferases EZH2 EZH2 enhancer of zeste 2 polycomb repressive complex 2 subunit Methyltransferases SUV39H1 SUV39H1 suppressor of variegation 3-9 homolog 1 (Drosophila) Methyltransferases SUV39H2 SUV39H2 suppressor of variegation 3-9 homolog 2 (Drosophila) Methyltransferases SET7/9 SET7/9 SET domain containing (lysine methyltransferase) 7 Methyltransferases SETD8 SETD8 SET domain containing (lysine methyltransferase) 8 Methyltransferases MLL SEPT9 septin 9 Methyltransferases CARM1 CARM1 coactivator-associated arginine methyltransferase 1 Methyltransferases PRMT1 PRMT1 protein arginine methyltransferase 1 Methyltransferases PRMT3 PRMT3 protein arginine methyltransferase 3 Methyltransferases PRMT5 PRMT5 protein arginine methyltransferase 5 Methyltransferases LSD1 KDM1A  lysine (K)-specific demethylase 1A Demethylases JMJD1A KDM3A lysine (K)-specific demethylase 3A Demethylases JMJD2A KDM4A lysine (K)-specific demethylase 4A Demethylases JMJD2B KDM4B lysine (K)-specific demethylase 4B Demethylases JMJD2C KDM4C lysine (K)-specific demethylase 4C Demethylases JMJD2D KDM4D lysine (K)-specific demethylase 4D Demethylases JMJD2E KDM4E lysine (K)-specific demethylase 4E Demethylases JMJD3 KDM6B lysine (K)-specific demethylase 6B Demethylases FBXL10 KDM2B lysine (K)-specific demethylase 2B Demethylases FBXL11 KDM2A lysine (K)-specific demethylase 2A Demethylases Available Epigentic Assays Nanosyn Name Symbol Approved Name Group Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 10. nanosyn.com Accelerating your drug discovery PDE1A1 PDE1A1 phosphodiesterase 1A, calmodulin-dependent PDE1B  PDE1B  phosphodiesterase 1B, calmodulin-dependent PDE1C  PDE1C  phosphodiesterase 1C, calmodulin-dependent 70kDa PDE2A  PDE2A  phosphodiesterase 2A, cGMP-stimulated PDE3A  PDE3A  phosphodiesterase 3A, cGMP-inhibited PDE3B  PDE3B  phosphodiesterase 3B, cGMP-inhibited PDE4A1A  PDE4A phosphodiesterase 4A, cAMP-specific PDE4B1  PDE4B phosphodiesterase 4B, cAMP-specific PDE4B2  PDE4C1  PDE4C phosphodiesterase 4C, cAMP-specific PDE4D2  PDE4D3  PDE4D7 PDE5A1  PDE5A phosphodiesterase 5A, cGMP-specific Available PDE Assays Nanosyn Name Symbol Approved Name Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 11. CD45 PTPRC protein tyrosine phosphatase, receptor type, C LAR PTPRF protein tyrosine phosphatase, receptor type, F PTP-Beta PTPRB protein tyrosine phosphatase, receptor type, B PTP-MU PTPRM  protein tyrosine phosphatase, receptor type, M PEST PCNP PEST proteolytic signal containing nuclear protein SHP-1 NR0B2 nuclear receptor subfamily 0, group B, member 2 PTP1B PTPN1 protein tyrosine phosphatase, non-receptor type 1 TC-PTP PTPN2 protein tyrosine phosphatase, non-receptor type 2 PTP-D2 PTGDR2 prostaglandin D2 receptor 2 PTEN PTEN phosphatase and tensin homolog VHR DUSP3 dual specificity phosphatase 3 SHIP2 INPPL1 inositol polyphosphate phosphatase-like 1 MEG1 MEG2 BAS LMPTP-A PP2AC nanosyn.com Available Phosphate Assays Nanosyn Approved Symbol Approved Name Accelerating your drug discovery Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com
  • 12. Aspartic BACE1 beta-site APP-cleaving enzyme 1 Aspartic CTSD CATHEPSIN D Cysteine CAPN1 Calpain-1 Cysteine CASP1 CASPASE 1 Cysteine CASP2 CASPASE 2 Cysteine CASP3 CASPASE 3 Cysteine CASP5 CASPASE 5 Cysteine CASP6 CASPASE 6 Cysteine CASP7 CASPASE 7 Cysteine CASP8 CASPASE 8 Cysteine CASP9 CASPASE 9 Cysteine CTSB CATHEPSIN B Cysteine CTSK CATHEPSIN K Cysteine CTSL CATHEPSIN L Cysteine CTSS CATHEPSIN S Metallo ACE angiotensin I converting enzyme Metallo ACE2 "angiotensin I converting enzyme 2" Metallo ADAM10 ADAM metallopeptidase domain 10 Metallo DPP3 dipeptidyl-peptidase 3 Metallo IDE insulin-degrading enzyme Metallo MMP1 matrix metallopeptidase 1 Metallo MMP12 matrix metallopeptidase 12 Metallo MMP13 matrix metallopeptidase 13 Metallo MMP14 matrix metallopeptidase 14 (membrane-inserted) Metallo MMP2 matrix metallopeptidase 2 Metallo MMP3 matrix metallopeptidase 3 Metallo MMP7 matrix metallopeptidase 7 Metallo MMP8 matrix metallopeptidase 8 Metallo MMP9 matrix metallopeptidase 9 Metallo MME membrane metallo-endopeptidase (NEPRILYSIN) Metallo ADAM17 ADAM metallopeptidase domain 17 (TACE) Serine DPP4 dipeptidyl-peptidase 4 Serine DPP8 dipeptidyl-peptidase 8 Serine DPP9 dipeptidyl-peptidase 9 Serine FACTOR Xa FACTOR Xa Serine FAP fibroblast activation protein, alpha Serine FURIN furin (paired basic amino acid cleaving enzyme) Serine GZMA granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) Serine GZMB granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) Serine GZMK granzyme K (granzyme 3; tryptase II) Serine Plasma Kallikrein Plasma Kallikrein Serine Plasmin Plasmin Serine Prolyl Oligopeptidase Prolyl Oligopeptidase Serine THROMBIN THROMBIN Serine tPA tPA Serine UPA UPA Serine HTRA2 HtrA serine peptidase 2 Serine ClpP/X caseinolytic mitochondrial matrix peptidase proteolytic subunit Threonine Proteasome Chymo-trypsin like Chymo-trypsin like Threonine Proteasome trypsin like Proteasome trypsin like PSMB8 proteasome (prosome, macropain) subunit, beta type, 8 Immuno-proteasome trypsin like death-associated protein kinase 1 nanosyn.com Accelerating your drug discovery Available Protease Assays Group Symbol Approved Name Olga Issakova Executive Vice President (408) 987-2004 oissakova@nanosyn.com

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