Polymer monolith prekubator tto
Published on: Mar 4, 2016
Transcripts - Polymer monolith prekubator tto
POLYMER MONOLITHIC COLUMN AND PREPARATION TECHNOLOGYKeywords: Liquid Chromatography, monolithic column, peptide separation, bioinformatics, lab-on-a-chip,diagnostic arrayBackgroundThe Achilles heel of bottom-up proteomics is the overwhelming number of peptides that needto be separated and identified using liquid chromatography coupled with tandem massspectrometry (LC-MS/MS). Sensitivity can be improved by using chromatography columnswith low internal diameter (ID). Because of the difficulty in filling a very narrow capillary tubewith particles, commercially available packed columns are technologically limited to 0.075 mmID.DescriptionIn theory, there is no such limitation to preparation of monolithic columns. Still, there are fewmonolithic products available, and typically the ID’s of these are 0.1 mm and higher. Thepreparation of very narrow ID monolithic columns is difficult. The product is a preparationtechnology (the ingredients - monomers, porogens and initiator - and the preparation setup) ofpolymer monolithic liquid chromatography column with very low internal diameter (0.03 mm)for sensitive nanoscale peptide separations. Figure BPI chromatogram of peptides from CSF tryptic digest using polymeric monolith and trap-columnApplications and benefits- Very low internal diameter (ID is between 0,05mm and 0,02mm)- Easy to reproduce – lower preparation costs, time and difficulty- Opportunities for both clinical diagnostic and R&DStatusVerified and tested prototypeIPRPatent pendingWe are seeking licensing partners.