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Pressure BioSciences - PCT News - August 2011

Pressure BioSciences August 2011 Newsletter
Published on: Mar 4, 2016
Published in: Technology      Business      
Source: www.slideshare.net

Transcripts - Pressure BioSciences - PCT News - August 2011

  • 1. PCT News NASDAQ: PBIO Discovery Starts With Sample Preparation TMVolume 5, Issue 8 August 2011 Pressure BioSciences’ Dr. Alexander Lazarev (VP of R&D) Presents Poster and Podium Talk Featuring the Novel & New Barocycler HUB440 at the 49th European High Pressure Research Group (EHPRG) Conference Program also Included Presentations by Scientists Using Pressure Cycling Technology (PCT) from the Barnett Institute of Northeastern University, and from the University of Massachusetts - Boston European High Pressure Research European High Pressure Research Group (EHPRG) Conference Group (EHPRG) Conference Podium Talk Poster Presentation Dr. Alex Lazarev Pressure BioSciences Trends and Applications of Hydrostatic Development and Applications of an Pressure in Biomedical Analysis: Exploring Automated, Benchtop, Dynamic a Forgotten Thermodynamic Dimension Pressure Generator for High Pressure Bioscience Research Alexander Lazarev, Timothy Straub, James Behnke and Edmund Y. Ting Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375, USA Pressure is a significant thermodynamic parameter that is orthogonal to temperature. The work of the 1946 Nobel Prize winner Percy W. Bridgman inspired further research of high pressure thermodynamics in biological systems. However, the limitations of material science and engineering to these days have led to a prominent lack of commercial laboratory equipment suitable to adequately support high pressure research efforts in life sciences. For decades, high pressure research has been driven by do-it-yourself CLICK HERE FOR THE COMPLETE enthusiasts capable of combining fundamental PRESENTATION knowledge of biology, physics and chemistry with the need to practice general plumbing and sophisticated mechanical engineering skills. Majority of the high pressure biochemistry and PBI EVENTS biophysics work has been done using primitive PBI to showcase pressure cycling technology manual equipment and hours of mandatory (PCT) and its applications in forensics at the physical exercise. The goal of this work is to Midwestern Association of Forensics Scientists develop a user-friendly, dynamically-controlled, (MAFS) meeting in Lombard, IL on September 19-23, 2011. Mr. Tom Strader (Sales Director, automated pressure generator suitable for Midwest) will present a summary of research unattended pressure perturbation studies of results generated at PBI and other laboratories protein conformation, including enzymology, using PBI’s Barocycler that show improvement kinetics of protein folding, aggregation, and self- in the extraction of DNA from forensic samples. assembly. Continued on Page 2 Column 1 www.pressurebiosciences.com
  • 2. PCT News NASDAQ: PBIO Discovery Starts With Sample Preparation TMVolume 5, Issue 8 August 2011Development and Applications of the Automated BenchtopDynamic Pressure Generator for High Pressure Bioscience European High Pressure ResearchResearch: Continued from Page 1, Column 2 Group (EHPRG) ConferenceHUB440 Pressure Generator: Flow Diagram Podium Presentation Barnett Institute, Water Inlet Northeastern University Filter Check Valve 60,000 PSI MAX Pressure Power 30 KPSI Out Pressure HP Air Pressure Check Valve HP “T” FittingPressureSource Display Inlet Press. HIGH PRESSURE MEDIATED SAMPLE Regulator(Air) Power indicator 160 PSI MAX Shift valve 0 60 KPSI PREPARATION FOR CAPILLARYExternal Control System Shift Valve Error Sensor Error indicator ELECTROPHORESIS ANALYSIS OFComputerControl Extend Intensifier 440:1 Intensifier N-LINKED GLYCANS Command Pressure András Guttman1, 2, Zoltán Szabó1 and Barry L. Karger1 1 Barnett Display indicator Institute, Northeastern University, Boston, MA 02115 2 HLBS, University of Debrecen, HungaryThe core of the system is an electronically- Effect of the PNGase F concentration on N-deglycosylation efficiency under PCT vs. atmospheric reaction conditionscontrolled air-driven reciprocating pressure PNGase F : Substrate ratio 1:10000 1:5000 1:2500 1:666 1:1250intensifier with a fixed ratio of 1:440, with a singlestroke displacement volume of 3.6 mL and a PCT (% intact RNase B) 83 71 7 7 7maximum outlet pressure of 4 kBar. The intensifier Atmospheric (% intact RNase B) 84 69 20 7 8 ois equipped with a dynamic, high pressure seal Pressure cycle level:20 kPsi, Reaction time:20 min., Temperature:37 C.system with minimum friction, resulting in theability to ramp pressure over time with a resolution Advantages of pressure cycling technologyof several Bar per second in both ascending and (PCT) assisted enzymatic N-deglycosylationdescending directions. Water is used as a highpressure media due to its low compressibility. An • The high pressure facilitates conformationelectronic regulator controls the air pressure and changes of the target glycoprotein,the direction of air flow to the front or the back of increasing the accessibility of thethe air cylinder, resulting in a precise extension or endoglycosidase to the cleavage sites.retraction of the intensifier piston. Pressure is • 1:2500 enzyme:substrate molar ratio atcontrolled via a manual control panel on the front 30 kPsi and 37oC quantitatively releasedof the instrument or remotely by the USB-powered the asparagine linked glycans in minutes.Data Acquisition and Control interface. A built-inhigh pressure transducer permits real-time • Pressure cycling apparently did not lead topressure feedback. The system is powered by a 24 any loss of sialic acid residues.VDC power supply. High pressure check valves • The microliter scale reaction volumeprevent the back flow of water and enable rapid alleviated possible precipitation relatedrefill of the intensifier with water for multi-stroke issues.operation. Open frame packaging of the • PCT offers simultaneous processing of 12instrument provides easy access to all serviceable samples.components and enables rapid customization. Ahigh pressure outlet is equipped with the standard CLICK HERE FOR THEhigh pressure type “T” fitting compatible with a COMPLETEwide variety of industry-standard high pressure Presentationequipment. CLICK HERE FOR THE COMPLETE POSTER www.pressurebiosciences.com
  • 3. PCT News NASDAQ: PBIO Discovery Starts With Sample Preparation TMVolume 5, Issue 8 August 2011 European High Pressure Research Environmentally Benign Reduction of Organic Compounds in Water under High Hydrostatic Pressure: Group (EHPRG) Conference Continued from Page 3,Column 1 Podium Presentation Conclusion: University of Massachusetts Boston • Development of “green” reduction method for CENTER FOR GREEN CHEMISTRY broad variety of ketones Environmentally Benign Reduction • Raney Ni-Al alloy, water, ketone & high of Organic Compounds in Water under hydrostatic pressure High Hydrostatic Pressure 1 2 Anna Tomin , Alexander Lazarev , and Bela Torok 1 2 1 • In situ generated hydrogen – remained UMASS Boston and Pressure BioSciences, Inc . in the solution Green Chemistry works toward sustainability by: • Corresponding products (alcohols, • Making chemical products that do not harm hydrocarbon, alkene) – good yield & either our health or the environment, selectivity CLICK HERE FOR THE • Using industrial processes that reduce or COMPLETE eliminate hazardous chemicals, and PRESENTATiON • Designing more efficient processes that minimize the production of waste materials. P.J. Dunn, A.S. Wells, M.T. Williams, Green Chemistry in the Pharmaceutical Industry, 2010., Wiley--VCH Our aims  Develop an environmentally benign process:  Reduction of ketones using 50-50% Ni-Al alloy in Water under high hydrostatic pressure (40,000 psi)  Extend the process for a broad scope of ketones www.pressurebiosciences.com

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