U g e l s t a d
L a b o r a t o r y
Nanomaterials for Drug
Delivery
Sulalit
PhD Stipendiat
Ugelstad Laboratory, Dept. o...
Roadmap
Drug pathway
Golden rules
Developments on Layer by layer (LBL) approach
Developments on hydrogels
Future Work
2
E. Markovsky et al. / Journal of Controlled
Release 161 (2012) 446–460
3
Golden Rules
 Monodisperse Nanoparticle (NP) population
 Biocompatibility
 Long circulation times
 Target specific
 D...
LBL approach - outline
(a)
(b)
Ag
 Sodium citrate synthesis with reflux
 Poly Lysine (PL) coating
BB
B
 Rat PGP s...
QCM Results
Na-citrate baseline
∆f (Hz)
PL addition
siRNA addition
Time (s)
Sauerbrey equation:
∆mPL
= 8533 ng cm-2
∆...
LBL approach – preliminary results
(a)
(b)
- PGP siRNA
- Ctrl siRNA
PGP silencing in vitro
~66nm Ag-PL NPs (DLS)
+33 mV...
Precipitation polymerization
- Monomer + cross linking agent in water
-T>LCST
-Homogeneous nucleation on collapsed oligom...
 Sythesizing
PNIPAm
Re-crystallization 
Size of PNIPAm at different concentrations
Size
(nm)
LCST
Temperature (°C)
10
PNIPAm concentration of 14.73 mg/mL
Size
(nm)
LCST
Temperature (°C)
11
Size of PNIPAm/AAc at different concentrations
Size
(nm)
VPTT
Temperature (°C)
12
The road ahead!
 Optimization of Au-PNIPAM-PAAC hydrogels
 PGP silencing studies
 EM setup for release studies
 Drug R...
14
of 14

Nanomaterials for Drug Delivery

Group Meeting, 18th November, 2013 (Monday)
Published on: Mar 3, 2016
Published in: Technology      Business      
Source: www.slideshare.net


Transcripts - Nanomaterials for Drug Delivery

  • 1. U g e l s t a d L a b o r a t o r y Nanomaterials for Drug Delivery Sulalit PhD Stipendiat Ugelstad Laboratory, Dept. of Chem. Eng., NTNU 18th November, 2013
  • 2. Roadmap Drug pathway Golden rules Developments on Layer by layer (LBL) approach Developments on hydrogels Future Work 2
  • 3. E. Markovsky et al. / Journal of Controlled Release 161 (2012) 446–460 3
  • 4. Golden Rules  Monodisperse Nanoparticle (NP) population  Biocompatibility  Long circulation times  Target specific  Delivery of cargo 4
  • 5. LBL approach - outline (a) (b) Ag  Sodium citrate synthesis with reflux  Poly Lysine (PL) coating BB B  Rat PGP siRNA and negative control siRNA 5
  • 6. QCM Results Na-citrate baseline ∆f (Hz) PL addition siRNA addition Time (s) Sauerbrey equation: ∆mPL = 8533 ng cm-2 ∆msiRNA = 2854 ng cm-2 6
  • 7. LBL approach – preliminary results (a) (b) - PGP siRNA - Ctrl siRNA PGP silencing in vitro ~66nm Ag-PL NPs (DLS) +33 mV (a) 427nm Absorbance ~38nm Ag NPs (DLS) , -45 mV (b) 432nm Wavelength (nm) 7
  • 8. Precipitation polymerization - Monomer + cross linking agent in water -T>LCST -Homogeneous nucleation on collapsed oligomers -Oligomer and monomer addition, aggregation Low PDI, control of charge,size, cross-link density 8
  • 9.  Sythesizing PNIPAm Re-crystallization 
  • 10. Size of PNIPAm at different concentrations Size (nm) LCST Temperature (°C) 10
  • 11. PNIPAm concentration of 14.73 mg/mL Size (nm) LCST Temperature (°C) 11
  • 12. Size of PNIPAm/AAc at different concentrations Size (nm) VPTT Temperature (°C) 12
  • 13. The road ahead!  Optimization of Au-PNIPAM-PAAC hydrogels  PGP silencing studies  EM setup for release studies  Drug Release Kinetics  Shape effect in coating 13
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